Artigos Científicos


Loth K, Vergnes A, Barreto C, Voisin S, Meudal H, Silva J, Bressan A, Belmadi N, Bachère E, Aucagne V, Cazevieille C, Marchandin H, Rosa RD, Bulet P, Touqui L, Delmas A, Destoumieux-Garzón D (2019) The ancestral N-terminal domain of big defensins drives bacteria-triggered assembly into antimicrobial nanonets. mBio, 10: e01821-19.

Cantelli L, Goncalves P, Guertler C, Kayser M, Pilotto MR, Barracco MA, Perazzolo LM (2019) Dietary supplementation with sulfated polysaccharides from Gracilaria birdiae promotes a delayed immunostimulation in marine shrimp challenged by white spot syndrome virus. Aquaculture International, 27: 349-367.

Pilotto MR, Milanez S, Moreira RT, Rosa RD, Perazzolo LM (2019) Potential immunomodulatory and protective effects of the Arthrospira-based dietary supplement on shrimp intestinal immune defensesFish and Shellfish Immunology, 88: 47-52.

Farias ND, Falchetti M, Matos GM, Schmitt P, Barreto C, Argenta N, Rolland JL, Bachère E, Perazzolo LMRosa RD (2019) Litopenaeus vannamei stylicins are constitutively produced by hemocytes and intestinal cells and are differentially modulated upon infectionsFish and Shellfish Immunology, 86: 82-92.



Matos GM, Schmitt P, Barreto C, Farias ND, Toledo-Silva G, Guzmán F, Destoumieux-Garzón D, Perazzolo LM, Rosa RD (2018) Massive gene expansion and sequence diversification is associated with diverse tissue distribution, regulation and antimicrobial properties of anti-lipopolysaccharide factors in shrimp. Marine Drugs, 16: 381.

Rode MP, Batti Angulski A, Gomes FA, Silva MM, Jeremias TDS, Carvalho RG, Iucif Vieria DG, Oliveira LFC, Fernandes Maia L, Trentin AG,  Hayashi L, Miranda KR, Aguiar AK, Rosa RD, Calloni GW (2018) Carrageenan hydrogel as a scaffold for skin-derived multipotent stromal cells deliveryJournal of Biomaterials Applications, 33: 422-434.

Pilotto MR, Goncalves ANA, Vieira FN, Seifert WQ, Bachère E, Rosa RD, Perazzolo LM (2018) Exploring the impact of the biofloc rearing system and an oral WSSV challenge on the intestinal bacteriome of Litopenaeus vannamei. Microorganisms, 6: 83.

Silveira AS, Matos GM, Falchetti M, Ribeiro FS, Bressan A, Bachère E, Perazzolo LM, Rosa RD (2018) An immune-related gene expression atlas of the shrimp digestive system in response to two major pathogens brings insights into the involvement of hemocytes in gut immunity. Developmental and Comparative Immunology, 79: 44-50.

Barreto C, Coelho JR, Yuan J, Xiang JH, Perazzolo LM, Rosa RD (2018) Specific molecular signatures for Type II crustins in penaeid shrimp uncovered by the identification of crustin-like antimicrobial peptides in Litopenaeus vannamei. Marine Drugs, 16: 31.



Kalil S, Rosa RD, Capelli-Peixoto J, Pohl PC, Oliveira PL, Fogaça AC, Daffre S (2017) Immune-related redox metabolism of embryonic cells of the tick Rhipicephalus microplus (BME26) in response to infection with Anaplasma marginale. Parasites and Vectors, 10: 613.

Vieira GC, da Silva PM, Barracco MA, Hering AF, Albuquerque MCP, Coelho JR, Schmidt EC, Bouzon ZL, Rosa RD, Perazzolo LM (2017)  Morphological and functional characterization of the hemocytes from the pearl oyster Pteria hirundo and their immune responses against Vibrio infectionsFish and Shellfish Immunology, 70: 750-758.

Ferreira AL, Maggioni R, Conceição D, Perazzolo LM, Petersen RL (2017) Hsp70 gene polymorphisms in farmed marine shrimp Litopenaeus vannamei populations exposed to white spot disease and infectious myonecrosis. Genetics and Molecular Research, 16: 2.



Quispe RL, Justino EB, Vieira FN, Jaramillo ML, Rosa RD, Perazzolo LM (2016) Transcriptional profiling of immune-related genes in Pacific white shrimp (Litopenaeus vannamei) during ontogenesisFish and Shellfish Immunology, 58: 103-107.

Coelho JR, Barreto C, Silveira AS, Vieira GC, Rosa RD, Perazzolo LM (2016) A hemocyte-expressed fibrinogen-related protein gene (LvFrep) from the shrimp Litopenaeus vannamei: expression analysis after microbial infection and during larval development. Fish and Shellfish Immunology, 56: 123-126.

Galletti MFBM, Fujita A, Rosa RD, Martins LA, Soares HS, Labruna MB, Daffre S, Fogaca AC (2016) Virulence genes of Rickettsia rickettsii are differentially modulated by either temperature upshift or blood feeding in tick midgut and salivary glands. Parasites and Vectors, 9: 331.

Alvarez C, Santana P, Donoso F, Ramírez F, Cortés J, Guzmán F, Rosa RD, Mercado L (2016) Approximation of immune response abilities in salmonid gills using the RTgill-W1 cell line as an in vitro modelFish and Shellfish Immunology, 53: 74.

Destoumieux-Garzón D, Rosa RD, Schmitt P, Barreto C, Vidal-Dupiol J, Mitta M, Gueguen Y, Bachère E (2016) Antimicrobial peptides in marine invertebrate health and disease. Philosophical Transactions of the Royal Society B, 371: 20150300.

Oyanedel D, Gonzalez R, Flores-Herrera P, Brokordt K, Rosa RD, Mercado L, Schmitt P (2016) Molecular characterization of an inhibitor of NF-κB in the scallop Argopecten purpuratus: first insights into its role on antimicrobial peptide regulation in a molluskFish and Shellfish Immunology, 52: 85-93.

Schmitt P, Rosa RD, Destoumieux-Garzón D (2016) An intimate link between antimicrobial peptide sequence diversity and binding to essential components of bacterial membranesBiochimica and Biophysica Acta (BBA) Biomembranes, 1858: 958-970.

Lehmann M, Schleder DD, Guertler C, Perazzolo LM, Vinatea LA (2016) Hypoxia increases susceptibility of Pacific white shrimp to white spot syndrome virus (WSSV)Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 68, p. 397-403.

Rosa RD, Capelli-Peixoto J, Mesquita RD, Kalil S, Pohl PC, Braz GR, Fogaça AC, Daffre S (2016) Exploring the immune signalling pathway-related genes of the cattle tick Rhipicephalus microplus: from molecular characterization to transcriptional profile upon microbial challenge. Developmental and Comparative Immunology, 59: 1-14



Bachère E, Rosa RD, Schmitt P, Poirier A, Merou N, Charrière G, Destoumieux-Garzón D (2015) The new insights into the oyster antimicrobial defense: cellular, molecular and genetic view. Fish and Shellfish Immunology, 46: 50-64.

Lehmann M, Schleder DD, Jatobá A, Perazzolo LM, Vinatea LA (2015) Suscetibilidade do camarão-branco do Pacífico à infecção com Vibrio alginolyticus em diferentes saturações de oxigênio. Agropecuária Catarinense, 28:67-70.

Feijó RG, Maggioni R, Martins PCC, de Abreu KL, Oliveira-Neto JM, Guertler C, Justino EB, Perazzolo LM, Marins LF (2015) RNAi-based inhibition of infectious myonecrosis virus replication in Pacific white shrimp Litopenaeus vannamei. Diseases of Aquatic Organisms, 114: 89-98.

Simões E, Vieira RC, Schramm M, Mello DF, Pontinha VC, da Silva PM, Barracco MA (2015) Impact of harmful algal blooms (Dinophysis acuminata) on the immune system of oysters (Crassostrea gigas) and mussels (Perna perna) cultivated in Santa Catarina, Brazil. Journal of the Marine Biological Association of the United Kingdom, 95: 773-781.

Rosa RD, Alonso P, Santini A, Vergnes A, Bachère E (2015) High polymorphism in big defensin gene expression reveals presence-absence gene variability (PAV) in the oyster Crassostrea gigasDevelopmental and Comparative Immunology, 49: 231-238.



Poirier A, Schmitt P, Rosa RD, Vanhove A, Kieffer-Jaquinod S, Charrière G, Destoumieux-Garzón D (2014) Antimicrobial histones and DNA traps in invertebrate immunity: evidences in Crassostrea gigas. The Journal of Biological Chemistry, 289: 24821-24831.

Goncalves P, Guertler C, Bachère E, de Souza CRB, Rosa RD, Perazzolo LM (2014) Molecular signatures at imminent death: hemocyte gene expression profiling of shrimp succumbing to viral and fungal infections. Developmental and Comparative Immunology, 42: 294-301.



Mello DF, da Silva PM, Barracco MA, Soudant P, Hégaret H (2013) Effects of the dinoflagellate Alexandrium minutum and its toxin (saxitoxin) on the functional activity and gene expression of Crassostrea gigas hemocytes. Harmful Algae, 26: 45-51.

Guertler C, Reig T, Mejía-Ruíz CH, Lehmann M, Barracco MA, Perazzolo LM (2013) Hemograma e sobrevivência de camarões marinhos após silenciamento do WSSV por RNA de interferência. Pesquisa Agropecuária Brasileira, 48: 983-990.

Rosa RD, Vergnes A, de Lorgeril J, Goncalves P, Perazzolo LM, Sauné L, Romestand B, Fievet J, Gueguen Y, Bachère E, Destoumieux-Garzón D (2013) Functional divergence in shrimp anti-lipopolysaccharide factors (ALFs): from recognition of cell wall components to antimicrobial activityPLoS ONE, 8: e67937.

Sabry RC ; Gesteira TCV, Magalhães ARM, Barracco MA, Guertler C, Ferreira LP, Vianna RT, da Silva PM (2013) Parasitological survey of mangrove oyster, Crassostrea rhizophorae, in the Pacoti River Estuary, Ceará State, Brazil. Journal of Invertebrate Pathology, 112: 24-32.



Goncalves P, Vernal J, Rosa RD, Yepiz-Plascencia G, de Souza CRB, Barracco MA, Perazzolo LM (2012) Evidence for a novel biological role for the multifunctional beta-1,3-glucan binding protein in shrimp. Molecular Immunology, 51: 363-367.

Rosa RD, de Lorgeril J, Tailliez P, Bruno R, Piquemal D, Bachère E (2012) A hemocyte gene expression signature correlated with predicted capacity of oysters to survive Vibrio infections. BMC Genomics, 13: 252.

Mello DF, de Oliveira ES, Vieira RC, Simões E, Trevisan R, Dafre AL, Barracco MA (2012) Cellular and transcriptional responses of Crassostrea gigas hemocytes exposed in vitro to brevetoxin (PbTx-2). Marine Drugs, 10: 583-597.

Schmitt P, Rosa RD, Duperthuy M, de Lorgeril J, Bachère E, Destoumieux-Garzón D (2012). The antimicrobial defense of the Pacific oyster, Crassostrea gigas. How diversity may compensate for scarcity in the regulation of resident/pathogenic microflora. Frontiers in Microbiology, 3: 160.

da Silva PM, Magalhães ARM, Barracco MA (2012) Pathologies in commercial bivalve species from Santa Catarina State, southern Brazil. Journal of the Marine Biological Association of the United Kingdom, 92: 571-579.



Perazzolo LM, Bachère E, Rosa RD, Goncalves P, Andreatta ER, Daffre S, Barracco MA (2011) Alpha2-macroglobulin from an Atlantic shrimp: Biochemical characterization, sub-cellular localization and gene expression upon fungal challenge. Fish & Shellfish Immunology, 31: 938-943.

Rosa RD, Santini A, Fievet J, Bulet P, Destoumieux-Garzón D, Bachère E (2011). Big defensins, a diverse family of antimicrobial peptides that follows different patterns of expression in hemocytes of the oyster Crassostrea gigas. PLoS ONE, 6: e25594.

Duperthuy M, Schmitt P, Garzón E, Caro A, Rosa RD, Le Roux F, Lautredou-Audouy N, Got P, Romestand B, de Lorgeril J, Kieffer-Jacquinod S, Bachère E & Destoumieux-Garzón D (2011). Use of OmpU for attachment and invasion of Crassostrea gigas immune cells by the oyster pathogen Vibrio splendidus. PNAS, 108: 2993-2998.

de Lorgeril J, Zenagui R, Rosa RD, Piquemal D, Bachère E (2011) Whole transcriptome profiling of successful immune response to Vibrio infections in the oyster Crassostrea gigas by digital gene expression analysis. PLoS ONE, 6: e23142.

Braunig P, Rosa RD, Seibert CH, Borsa M, Stoco PH, Grisard EC, Pinto AR (2011). Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a Brazilian white spot syndrome virus isolate. Brazilian Archives of Biology and Technology, 54: 399-404.

Borsa M, Seibert CH, Rosa RD, Stoco PH, Cargnin-Ferreira E, Pereira AML, Grisard EC, Zanetti CR, Pinto AR (2011) Detection of infectious myonecrosis virus in penaeid shrimps using immunoassays: usefulness of monoclonal antibodies directed to the viral major capsid protein. Archives of Virology, 156: 9-16.



Guertler C, Schleder DD, Barracco MA, Perazzolo LM (2010) Comparative study of the intracellular superoxide anion production in different penaeid species through the NBT-reduction assay. Aquaculture Research, 41: 1082-1088.

Seibert CH, Gargioni R, Rosa RD, Perazzolo LM, Nazari EM, Ammar D, Zanetti CR, Pinto AR (2010) A novel monoclonal antibody that binds to hemocytes from shrimps and oyster. Hybridoma, 29: 161-167.

Vieira FN, Buglione-Neto CC, Mouriño JLP, Jatobá A, Martins ML, Schleder DD, Andreatta ER, Barracco MA, Vinatea LA (2010) Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 62: 631-638.

Seibert CH, Borsa M, Rosa RD, Cargnin-Ferreira E, Pereira AML, Grisard EC, Zanetti CR, Pinto AR (2010) Detection of major capsid protein of infectious myonecrosis virus in shrimps using monoclonal antibodies. Journal of Virological Methods, 169: 169-175.

Mello DF, Proença LAO, Barracco MA (2010) Comparative study of various immune parameters in three bivalve species during a natural bloom of Dinophysis acuminata in Santa Catarina Island, Brazil. Toxins, 2: 1166-1178.

Rosa RD, Barracco MA (2010) Antimicrobial peptides in crustaceans. Invertebrate Survival Journal, 7: 262-284.



Costa AM, Buglione CC, Bezerra FL, Martins PCC, Barracco MA (2009) Immune assessment of farm-reared Penaeus vannamei shrimp naturally infected by IMNV in NE Brazil. Aquaculture, 291: 141-146.

Sabry RC, Rosa RD, Magalhães ARM, Barracco MA, Gesteria TCV, da Silva PM (2009) First report of Perkinsus sp. infecting mangrove oysters Crassostrea rhizophorae from the Brazilian coast. Diseases of Aquatic Organisms, 88: 13-23.

Barracco MARosa RD (2009) On shrimp interferon. Aquaculture, 298: 182.

Barracco MA, Zillig C (2009) Parceiro de Charles Darwin. Scientific American Brasil, 84: 72-76.

da Silva PM, Cremonte F, Sabry RC, Rosa RD, Cantelli L, Barracco MA (2009) Presence and histopathological effects of the Parvatrema sp. (Digenea, Gymnophallidae) in the stout razor clam Tagelus plebeius (Bivalvia, Psammobiidae). Journal of Invertebrate Pathology, 102: 14-20.

Löfgren SE, Smânia A, Smânia EFA, Bachère E, Barracco MA (2009) Comparative activity and stability under salinity conditions of different antimicrobial peptides isolated from aquatic animals. Aquaculture Research, 41: 1-8.

Rosa RD, Stoco PH, Barracco MA (2008). Cloning and characterisation of cDNA sequences encoding for anti-lipopolysaccharide factors (ALFs) in Brazilian palaemonid and penaeid shrimps. Fish & Shellfish Immunology, 25: 693-696.

Löfgren SE, Miletti LC, Steindel M, Bachère E, Barracco MA (2008) Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPs) isolated from aquatic animals. Experimental Parasitology, 118: 197-202.

Rosa RD, Barracco MA (2008). Shrimp interferon is rather a portion of the mitochondrial F0-ATP synthase than a true alpha-interferon. Molecular Immunology, 45: 3490-3493.

Schleder DD, Kayser M, Sühnel S, Ferreira JF, Rupp GS, Barracco MA (2008) Evaluation of some hemato-immunological parameters during the reproductive cycle of the scallop Nodipecten nodosus in association with a carotenoid-enriched diet. Aquaculture, 280: 256 – 263.

Rosa RD, Perazzolo LM, Barracco MA (2008). Comparison of the thioester domain and adjacent regions of the alpha2-macroglobulin from different South Atlantic crustaceans. Fish & Shellfish Immunology, 24(2): 257-259.

Vieira FN, Buglione-Neto CC, Mouriño JLP, Jatobá A, Ramirez C, Martins ML, Barracco MA, vinatea LA (2008) Time-related action of Lactobacillus plantarum in the bacterial microbiota of shrimp digestive tract and its action as immunostimulant. Pesquisa Agropecuária Brasileira, 43: 763-769.

Rosa RD, Bandeira PT, Barracco MA (2007) Molecular cloning of crustins from the hemocytes of Brazilian penaeid shrimps. FEMS Microbiology Letters, 274: 287-290.

Carriel-Gomes MC, Kratz JM, Barracco MA, Bachère E, Barardi CRM, Simões CMO (2007) In vitro antiviral activity of antimicrobial peptides against herpes simplex virus 1, adenovirus, and rotavirus. Memórias do Instituto Oswaldo Cruz, 102: 469-472.

Gueguen Y, Garnier J, Robert L, Lefranc MP, Mougenot I, de Lorgeril J, Janech M, Gross PS, Warr GW, Cuthbertson B, Barracco MA, Bulet P, Aumelas A, Yang Y, Bo D, Xiang J, Tassanakajon A, Piquemal D, Bachère E (2006) PenBase, the shrimp antimicrobial peptide penaeidin database: Sequence-based classification and recommended nomenclature. Developmental and Comparative Immunology, 30: 283-288.

Barracco MA, de Lorgeril J, Gueguen Y, Bachère E (2005) Molecular characterization of penaeidins from two Atlantic shrimp species, Farfantepenaeus paulensis and Litopenaeus schmitti.  FEMS Microbiology Letters, 250: 117-120.

Perazzolo LM, Lorenzini DM, Daffre S,  Barraco MA (2005) Purification and characterization of the plasma clotting protein of the pink shrimp Farfantepenaeus paulensis. Comparative Biochemistry and Physiology Part B, 142: 302-307.

Barth T, Morais N, Barracco MA (2005) Evaluation of some hemato-immunological and histological parameters in the mangrove oyster Crassostrea rhizophorae in different habitats of Santa Catarina Island, Brazil. Aquatic Living Resources, 18: 179-186.

Maggioni DG, Andreatta ER, Hermes EM, Barracco MA (2004) Evaluation of some hemato-immunological parameters in female shrimp Litopenaeus vannamei submitted to unilateral eyestalk ablation in association with a diet supplemented with superdoses of ascorbic acid as a form of immunostimulation. Aquaculture, 241: 501-515.

Perazzolo LM, Gargioni R, Ogliari P, Barracco MA (2002) Evaluation of some hemato-immunological parameters in the shrimp Farfantepenaeus paulensis submitted to environmental and physiological stress. Aquaculture, 214: 19-33.

Cominetti MR, Marques MRF, Lorenzini DM, Löfgren SE, Daffre S, Barracco MA (2002) Characterization and partial purification of an agglutinating factor of the hemolymph of the white shrimp Litopenaeus schmitti. Developmental and Comparative Immunology, 26: 715-721.

da Silva PM, Magalhães ARM, Barracoo MA (2001) Effects of Bucephalus sp (Trematoda: Bucephalidae) on Perna perna mussels, from a culture station in Ratones Grande islandJournal of Invertebrate Pathology, 79: 154-162.

Marques MRF, Barracco MA (2000) Lectins, as non-self recognition factors, in crustaceans. Aquaculture, 191: 23-44.



Perazzolo LM, Coward K, Davail B, Normand E, Tyler CR, Pakdel F, Schneider WJ, Le Menn F (1999) Expression and localization of messenger ribonucleic acid for the vitellogenin receptor in ovarian follicles throughout oogenesis in the rainbow trout, Oncorhynchus mykiss. Biology of Reproduction, 60: 1057-1068.

Barracco MA, Medeiros ID, Moreira FM (1999) Some hemato-immunological parameters in the mussel Perna perna. Fish & Shellfish Immunology, 9: 387-404.

Davail B, Pakdel F, Bujo H, Perazzolo LM, Waclawek M, Schneider WJ, Le Menn (1998) Evolution of oogenesis: the receptor for vitellogenin from the rainbow trout. Journal of Lipid Research, 39: 1929-1937.

Gargioni R, Barracco MA (1998) Hemocytes of the palaemonids Macrobrachium rosenbergii and M. acanthurus, and of the penaeid Penaeus paulensis. Journal of Morphology, 236: 209-221.

Perazzolo LM, Barracco MA (1997) The prophenoloxidase activating system of the shrimp Penaeus paulensis and associated factors. Developmental and Comparative Immunology, 21: 385-395.

Barracco MA, Söderhäll K (1996) Effect of fixed epimastigote forms of Trypanosoma cruzi on the hemocytes and the phenoloxidase activating system of the crayfish Pacifastacus leniusculus. Brazilian Journal of Medical and Biological Research, 29: 1321- 1327.

Barracco MA, Steil AA, Gargioni R (1993) Morphological characterization of the hemocytes of the pulmonate snail Biomphalaria tenagophila. Memórias do Instituto Oswaldo Cruz, 88: 73-83.

Barracco MA, Amirante GA (1992) Morphological and cytochemical studies of the hemocytes of Squilla mantis (Stomatopoda). Journal of Crustacean Biology, 12: 372-382.

Barracco MA, Duvic B, Söderhäll K (1991) The beta-1,3-glucan binding protein from the crayfish Pacifastacus leniusculus, when reacted with a beta-1,3-glucan, induces spreading and degranulation of crayfish granular cells. Cell and Tissue Research, 266: 491-497.